Affinity chromatography is a method of separating a biomolecule from a mixture, based on a highly specific macromolecular binding interaction between the biomolecule and another substance.

Affinity chromatography is another powerful technique of purifying proteins. This technique takes advantage of the high affinity of many proteins for specific chemical groups.

Mar 31, 2025 · Affinity chromatography is a type of liquid chromatography, which utilizes the reversible biological interaction (affinity) between components in the mobile phase and a solid stationary phase …

Abstract The field of affinity chromatography, which employs a biologically-related agent as the stationary phase, has seen significant growth since the modern era of this method began in 1968. …

Nov 10, 2025 · Affinity chromatography is a specialized technique used to separate components in a mixture. Unlike other chromatographic methods that separate molecules based on general physical …

Affinity chromatography is a separation method based on a specific binding interaction between an immobilized ligand and its binding partner. Examples include antibody/antigen, enzyme/substrate, …

Dec 13, 2025 · Affinity chromatography is a type of liquid chromatography technique which is used for separation and purification of biomolecules based on a highly specific and reversible interaction …

Affinity chromatography (Fig. 5.8) is an affinity-based separation technique where the constituents of a mixture are separated based on their affinity toward a special tag/linker associated to the stationary …

Explore the fundamentals, ligands, and diverse applications of affinity chromatography in protein purification and drug discovery.

The most powerful of these methods is affinity chromatography, also called affinity purification, whereby the protein of interest is purified by virtue of its specific binding properties to an immobilized ligand.